Rat neutrophil elastase,NE ELISA Kit
Product Details
| Target Name |
neutrophil elastase,NE |
| Alternative Names |
Bone marrow serine protease ELISA Kit; ELA2 ELISA Kit; ELANE ELISA Kit; Elastase 2 ELISA Kit; Elastase 2 neutrophil ELISA Kit; Elastase neutrophil expressed ELISA Kit; Elastase-2 ELISA Kit; ELNE_HUMAN ELISA Kit; GE ELISA Kit; Granulocyte derived elastase ELISA Kit; HLE ELISA Kit; HNE ELISA Kit; Human leukocyte elastase ELISA Kit; Leukocyte elastase ELISA Kit; Medullasin ELISA Kit; NE ELISA Kit; Neutrophil elastase ELISA Kit; PMN E ELISA Kit; PMN elastase ELISA Kit; Polymorphonuclear elastase ELISA Kit; SCN1 ELISA Kit |
| Abbreviation |
NE |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates, cell lysates |
| Detection Range |
12.5 ng/mL-800 ng/mL |
| Sensitivity |
3.12 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat NE in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
89 |
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| Range % |
83-93 |
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| 1:2 |
Average % |
85 |
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| Range % |
80-90 |
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| 1:4 |
Average % |
91 |
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| Range % |
83-95 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-97 |
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| Recovery |
| The recovery of rat NE spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
97 |
92-102 |
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| EDTA plasma (n=4) |
95 |
90-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 800 |
2.805 |
2.767 |
2.786 |
2.584 |
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| 400 |
2.462 |
2.374 |
2.418 |
2.216 |
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| 200 |
2.131 |
2.089 |
2.110 |
1.908 |
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| 100 |
1.669 |
1.578 |
1.624 |
1.422 |
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| 50 |
1.213 |
1.156 |
1.185 |
0.983 |
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| 25 |
0.857 |
0.793 |
0.825 |
0.623 |
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| 12.5 |
0.446 |
0.424 |
0.435 |
0.233 |
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| 0 |
0.204 |
0.199 |
0.202 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
