| Target Name |
Orexin A |
| Alternative Names |
Hcrt ELISA Kit; Ox ELISA Kit; Ppox ELISA Kit; Orexin ELISA Kit; Hypocretin ELISA Kit; Hcrt) [Cleaved into: Orexin-A ELISA Kit; Hypocretin-1 ELISA Kit; Hcrt1); Orexin-B ELISA Kit; Hypocretin-2 ELISA Kit; Hcrt2)] ELISA Kit |
| Abbreviation |
Orexin A |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma |
| Detection Range |
15.6 pg/mL-1000 pg/mL |
| Sensitivity |
3.9 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Metabolism |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat orexin A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
93 |
|
| Range % |
84-107 |
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| 1:2 |
Average % |
92 |
|
| Range % |
86-99 |
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| 1:4 |
Average % |
95 |
|
| Range % |
91-99 |
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| 1:8 |
Average % |
96 |
|
| Range % |
90-103 |
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| Recovery |
| The recovery of rat orexin A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
96 |
89-101 |
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| EDTA plasma (n=4) |
88 |
83-95 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 1000 |
2.108 |
2.076 |
2.092 |
1.978 |
|
| 500 |
1.774 |
1.785 |
1.780 |
1.666 |
|
| 250 |
1.442 |
1.468 |
1.455 |
1.341 |
|
| 125 |
1.140 |
1.149 |
1.145 |
1.031 |
|
| 62.5 |
0.744 |
0.763 |
0.754 |
0.640 |
|
| 31.2 |
0.525 |
0.534 |
0.530 |
0.416 |
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| 15.6 |
0.291 |
0.282 |
0.287 |
0.173 |
|
| 0 |
0.113 |
0.114 |
0.114 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
