| Target Name |
selectin P (granule membrane protein 140kDa, antigen CD62) |
| Alternative Names |
Selp ELISA Kit; P-selectin ELISA Kit; CD62 antigen-like family member P ELISA Kit; Granule membrane protein 140 ELISA Kit; GMP-140 ELISA Kit; Leukocyte-endothelial cell adhesion molecule 3 ELISA Kit; LECAM3 ELISA Kit; Platelet activation dependent granule-external membrane protein ELISA Kit; PADGEM ELISA Kit; CD antigen CD62P ELISA Kit |
| Abbreviation |
SELP |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
9.38 ng/mL-600 ng/mL |
| Sensitivity |
2.34 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat P-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
93 |
|
| Range % |
85-99 |
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| 1:2 |
Average % |
92 |
|
| Range % |
84-105 |
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| 1:4 |
Average % |
97 |
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| Range % |
92-103 |
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| 1:8 |
Average % |
94 |
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| Range % |
82-98 |
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| Recovery |
| The recovery of rat P-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
97 |
95-100 |
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| EDTA plasma (n=4) |
95 |
92-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 600 |
2.755 |
2.812 |
2.784 |
2.616 |
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| 300 |
2.217 |
2.236 |
2.227 |
2.059 |
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| 150 |
1.545 |
1.524 |
1.535 |
1.367 |
|
| 75 |
1.051 |
0.979 |
1.015 |
0.847 |
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| 37.5 |
0.587 |
0.622 |
0.605 |
0.437 |
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| 18.75 |
0.456 |
0.438 |
0.447 |
0.279 |
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| 9.38 |
0.288 |
0.279 |
0.284 |
0.116 |
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| 0 |
0.173 |
0.162 |
0.168 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
