Rat Platelet-Derived Growth Factor A,PDGF-A ELISA kit
Product Details
| Target Name |
platelet-derived growth factor alpha polypeptide |
| Alternative Names |
Pdgfa ELISA Kit; Rpa1 ELISA Kit; Platelet-derived growth factor subunit A ELISA Kit; PDGF subunit A ELISA Kit; PDGF-1 ELISA Kit; Platelet-derived growth factor A chain ELISA Kit; Platelet-derived growth factor alpha polypeptide ELISA Kit |
| Abbreviation |
PDGFA |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, cell culture supernates |
| Detection Range |
0.312 pg/mL-20 pg/mL |
| Sensitivity |
0.078 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PDGF-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:20 |
Average % |
88 |
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| Range % |
84-92 |
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| 1:40 |
Average % |
94 |
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| Range % |
90-99 |
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| 1:80 |
Average % |
91 |
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| Range % |
85-96 |
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| 1:160 |
Average % |
96 |
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| Range % |
91-103 |
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| Recovery |
| The recovery of rat PDGF-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
98 |
94-102 |
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| EDTA plasma (n=4) |
103 |
99-107 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.469 |
2.603 |
2.536 |
2.421 |
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| 10 |
1.993 |
2.011 |
2.002 |
1.887 |
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| 5 |
1.451 |
1.397 |
1.424 |
1.309 |
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| 2.5 |
0.966 |
1.002 |
0.984 |
0.869 |
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| 1.25 |
0.584 |
0.554 |
0.569 |
0.454 |
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| 0.63 |
0.365 |
0.346 |
0.356 |
0.241 |
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| 0.312 |
0.255 |
0.260 |
0.258 |
0.143 |
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| 0 |
0.118 |
0.112 |
0.115 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
