Rat platelet-derived growth factor-BB,PDGF-BB ELISA Kit
Product Details
| Target Name |
platelet-derived growth factor-BB,PDGF-BB |
| Abbreviation |
PDGF-BB |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
39.063 pg/mL-2500 pg/mL |
| Sensitivity |
9.77 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PDGF-BB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
104 |
|
| Range % |
101-107 |
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| 1:2 |
Average % |
97 |
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| Range % |
94-100 |
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| 1:4 |
Average % |
88 |
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| Range % |
83-90 |
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| 1:8 |
Average % |
89 |
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| Range % |
84-93 |
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| Recovery |
| The recovery of rat PDGF-BB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
105 |
101-109 |
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| EDTA plasma (n=4) |
110 |
107-113 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2500 |
2.794 |
2.753 |
2.774 |
2.578 |
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| 1250 |
2.204 |
2.200 |
2.202 |
2.006 |
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| 625 |
1.530 |
1.501 |
1.516 |
1.320 |
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| 312.5 |
0.901 |
0.925 |
0.913 |
0.717 |
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| 156.25 |
0.635 |
0.652 |
0.644 |
0.448 |
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| 78.125 |
0.459 |
0.435 |
0.447 |
0.251 |
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| 39.063 |
0.376 |
0.368 |
0.372 |
0.176 |
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| 0 |
0.197 |
0.194 |
0.196 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
