Rat Platelet Factor 4,PF-4 ELISA Kit
Product Details
| Target Name |
platelet factor 4 |
| Alternative Names |
Pf4 ELISA Kit; Cxcl4 ELISA Kit; Scyb4 ELISA Kit; Platelet factor 4 ELISA Kit; PF-4 ELISA Kit; C-X-C motif chemokine 4 ELISA Kit |
| Abbreviation |
PF4 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.156 μg/mL-10 μg/mL |
| Sensitivity |
0.039 μg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PF-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
86-97 |
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| 1:2 |
Average % |
95 |
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| Range % |
88-99 |
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| 1:4 |
Average % |
95 |
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| Range % |
91-102 |
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| 1:8 |
Average % |
95 |
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| Range % |
87-104 |
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| Recovery |
| The recovery of rat PF-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
95 |
83-99 |
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| EDTA plasma (n=4) |
97 |
89-102 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| μg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 10 |
2.684 |
2.617 |
2.651 |
2.463 |
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| 5 |
2.204 |
2.186 |
2.195 |
2.007 |
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| 2.5 |
1.563 |
1.608 |
1.586 |
1.398 |
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| 1.25 |
0.881 |
0.899 |
0.890 |
0.702 |
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| 0.625 |
0.480 |
0.491 |
0.486 |
0.298 |
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| 0.312 |
0.345 |
0.337 |
0.341 |
0.153 |
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| 0.156 |
0.247 |
0.250 |
0.249 |
0.061 |
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| 0 |
0.185 |
0.190 |
0.188 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
