Rat Prostaglandin E2,PG-E2 ELISA Kit
Product Details
| Target Name |
Prostaglandin E2,PG-E2 |
| Abbreviation |
PG-E2 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates, cell lysates |
| Detection Range |
0.5 pg/mL-100 pg/mL |
| Sensitivity |
0.25 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Competitive |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat PG-E2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
96 |
|
| Range % |
91-99 |
|
| 1:2 |
Average % |
92 |
|
| Range % |
87-96 |
|
| 1:4 |
Average % |
93 |
|
| Range % |
89-98 |
|
| 1:8 |
Average % |
105 |
|
| Range % |
99-109 |
|
|
| Recovery |
| The recovery of rat PG-E2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
96 |
92-99 |
|
| EDTA plasma (n=4) |
101 |
96-104 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
|
|
| 100 |
0.228 |
0.224 |
0.226 |
|
|
| 25 |
0.365 |
0.348 |
0.357 |
|
|
| 8 |
0.560 |
0.539 |
0.550 |
|
|
| 2 |
0.813 |
0.787 |
0.800 |
|
|
| 0.5 |
1.258 |
1.234 |
1.246 |
|
|
| 0 |
2.031 |
2.176 |
2.104 |
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
