Rat prothrombin fragment 1+2,F1+2 ELISA Kit
Product Details
| Target Name |
prothrombin fragment 1+2,F1+2 |
| Abbreviation |
F1+2 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
62.5 pmol/L-4000 pmol/L |
| Sensitivity |
15.6 pmol/L |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8>
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10>
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat F1+2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
89 |
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| Range % |
80-92 |
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| 1:2 |
Average % |
100 |
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| Range % |
91-105 |
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| 1:4 |
Average % |
102 |
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| Range % |
92-110 |
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| 1:8 |
Average % |
93 |
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| Range % |
86-98 |
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| Recovery |
| The recovery of rat F1+2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
89-98 |
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| EDTA plasma (n=4) |
95 |
90-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pmol/L |
OD1 |
OD2 |
Average |
Corrected |
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| 4000 |
2.739 |
2.726 |
2.733 |
2.622 |
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| 2000 |
2.243 |
2.201 |
2.222 |
2.111 |
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| 1000 |
1.447 |
1.441 |
1.444 |
1.333 |
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| 500 |
0.802 |
0.739 |
0.771 |
0.660 |
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| 250 |
0.427 |
0.414 |
0.421 |
0.310 |
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| 125 |
0.269 |
0.252 |
0.261 |
0.150 |
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| 62.5 |
0.178 |
0.166 |
0.172 |
0.061 |
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| 0 |
0.113 |
0.109 |
0.111 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
