Rat Pulmonary Surfactant-associated protein B (SP-B) ELISA Kit
Product Details
| Target Name |
surfactant protein B |
| Alternative Names |
Sftpb ELISA Kit; Sftp3 ELISA Kit; Pulmonary surfactant-associated protein B ELISA Kit; SP-B ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Phe) ELISA Kit |
| Abbreviation |
SFTPB |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
3.12 pg/mL-200 pg/mL |
| Sensitivity |
0.78 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat SP-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1000 |
Average % |
90 |
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| Range % |
85-100 |
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| 1:2000 |
Average % |
94 |
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| Range % |
87-98 |
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| 1:4000 |
Average % |
90 |
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| Range % |
85-96 |
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| 1:8000 |
Average % |
94 |
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| Range % |
90-98 |
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| Recovery |
| The recovery of rat SP-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
96 |
90-101 |
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| EDTA plasma (n=4) |
91 |
85-97 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
2.470 |
2.589 |
2.530 |
2.377 |
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| 100 |
2.115 |
2.124 |
2.120 |
1.967 |
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| 50 |
1.713 |
1.726 |
1.720 |
1.567 |
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| 25 |
1.315 |
1.356 |
1.336 |
1.183 |
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| 12.5 |
0.985 |
0.971 |
0.978 |
0.825 |
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| 6.25 |
0.532 |
0.541 |
0.537 |
0.384 |
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| 3.12 |
0.378 |
0.389 |
0.384 |
0.231 |
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| 0 |
0.152 |
0.154 |
0.153 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
