Rat Pulmonary Surfactant-associated protein D (SP-D) ELISA kit
Product Details
| Target Name |
surfactant protein D |
| Alternative Names |
Sftpd ELISA Kit; Sftp4 ELISA Kit; Pulmonary surfactant-associated protein D ELISA Kit; PSP-D ELISA Kit; SP-D ELISA Kit; CP4 ELISA Kit; Lung surfactant protein D ELISA Kit |
| Abbreviation |
SFTPD |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.312 ng/mL-20 ng/mL |
| Sensitivity |
0.078 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat SP-D in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:5 |
Average % |
88 |
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| Range % |
82-92 |
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| 1:10 |
Average % |
94 |
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| Range % |
90-99 |
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| 1:20 |
Average % |
95 |
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| Range % |
91-101 |
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| 1:40 |
Average % |
94 |
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| Range % |
90-98 |
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| Recovery |
| The recovery of rat SP-D spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
89 |
85-96 |
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| EDTA plasma (n=4) |
92 |
87-98 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 20 |
2.689 |
2.697 |
2.693 |
2.508 |
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| 10 |
2.001 |
2.054 |
2.028 |
1.843 |
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| 5 |
1.555 |
1.579 |
1.567 |
1.382 |
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| 2.5 |
1.112 |
1.165 |
1.139 |
0.954 |
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| 1.25 |
0.687 |
0.697 |
0.692 |
0.507 |
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| 0.625 |
0.442 |
0.475 |
0.459 |
0.274 |
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| 0.312 |
0.382 |
0.396 |
0.389 |
0.204 |
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| 0 |
0.184 |
0.186 |
0.185 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
