Rat Soluble Cluster of differentiation 40 ligand,sCD40L ELISA Kit
Product Details
| Target Name |
CD40 ligand |
| Alternative Names |
Cd40lg ELISA Kit; Cd40l ELISA Kit; Tnfsf5CD40 ligand ELISA Kit; CD40-L ELISA Kit; Tumor necrosis factor ligand superfamily member 5 ELISA Kit; CD antigen CD154) [Cleaved into: CD40 ligand ELISA Kit; membrane form; CD40 ligand ELISA Kit; soluble form ELISA Kit; sCD40L)] ELISA Kit |
| Abbreviation |
CD40LG |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
1.87 ng/mL-120 ng/mL |
| Sensitivity |
0.47 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat sCD40L in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
95 |
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| Range % |
83-98 |
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| 1:2 |
Average % |
93 |
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| Range % |
87-98 |
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| 1:4 |
Average % |
91 |
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| Range % |
83-95 |
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| 1:8 |
Average % |
103 |
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| Range % |
95-107 |
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| Recovery |
| The recovery of rat sCD40L spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
88 |
82-92 |
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| EDTA plasma (n=4) |
95 |
90-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 120 |
2.487 |
2.688 |
2.588 |
2.437 |
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| 60 |
1.734 |
1.643 |
1.689 |
1.538 |
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| 30 |
1.049 |
1.106 |
1.078 |
0.927 |
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| 15 |
0.674 |
0.685 |
0.680 |
0.529 |
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| 7.5 |
0.442 |
0.457 |
0.450 |
0.299 |
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| 3.75 |
0.314 |
0.327 |
0.321 |
0.170 |
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| 1.87 |
0.236 |
0.245 |
0.241 |
0.090 |
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| 0 |
0.150 |
0.151 |
0.151 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
