Rat soluble E-selectin,sE-selectin ELISA Kit
Product Details
| Target Name |
selectin E |
| Alternative Names |
Sele ELISA Kit; Elam-1 ELISA Kit; E-selectin ELISA Kit; CD62 antigen-like family member E ELISA Kit; Endothelial leukocyte adhesion molecule 1 ELISA Kit; ELAM-1 ELISA Kit; Leukocyte-endothelial cell adhesion molecule 2 ELISA Kit; LECAM2 ELISA Kit; CD antigen CD62E ELISA Kit |
| Abbreviation |
SELE |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
78 pg/mL-5000 pg/mL |
| Sensitivity |
19.5 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat sE-selectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:5 |
Average % |
92 |
|
| Range % |
88-108 |
|
| 1:10 |
Average % |
94 |
|
| Range % |
91-98 |
|
| 1:20 |
Average % |
96 |
|
| Range % |
89-100 |
|
| 1:40 |
Average % |
84 |
|
| Range % |
80-88 |
|
|
| Recovery |
| The recovery of rat sE-selectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
99 |
93-105 |
|
| EDTA plasma (n=4) |
94 |
90-98 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 5000 |
2.746 |
2.645 |
2.696 |
2.542 |
|
| 2500 |
1.976 |
1.875 |
1.926 |
1.772 |
|
| 1250 |
1.228 |
1.204 |
1.216 |
1.062 |
|
| 625 |
0.782 |
0.761 |
0.772 |
0.618 |
|
| 312 |
0.522 |
0.511 |
0.517 |
0.363 |
|
| 156 |
0.363 |
0.352 |
0.358 |
0.204 |
|
| 78 |
0.290 |
0.285 |
0.288 |
0.134 |
|
| 0 |
0.154 |
0.153 |
0.154 |
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
