Rat soluble platelet endothelial cell adhesion molecule 1,sPECAM-1 ELISA Kit
Product Details
| Target Name |
platelet/endothelial cell adhesion molecule |
| Alternative Names |
Pecam1 ELISA Kit; Pecam ELISA Kit; Platelet endothelial cell adhesion molecule ELISA Kit; PECAM-1 ELISA Kit; CD antigen CD31 ELISA Kit |
| Abbreviation |
PECAM1 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
3.12 ng/mL-200 ng/mL |
| Sensitivity |
0.78 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cardiovascular |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV%<8> |
|
|
|
|
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
|
| Inter-assay Precision (Precision between assays): CV%<10> |
|
|
|
|
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
|
| |
|
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat sPECAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
|
| |
Sample |
Serum(n=4) |
|
|
| 1:1 |
Average % |
92 |
|
|
| Range % |
86-95 |
|
|
| 1:2 |
Average % |
102 |
|
|
| Range % |
97-107 |
|
|
| 1:4 |
Average % |
91 |
|
|
| Range % |
85-97 |
|
|
| 1:8 |
Average % |
97 |
|
|
| Range % |
91-103 |
|
|
|
| Recovery |
| The recovery of rat sPECAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
| |
| Sample Type |
Average % Recovery |
Range |
|
|
| Serum (n=5) |
95 |
89-98 |
|
|
| EDTA plasma (n=4) |
97 |
90-100 |
|
|
| |
|
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
| |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
|
| 200 |
2.468 |
2.554 |
2.511 |
2.372 |
|
|
| 100 |
1.656 |
1.732 |
1.694 |
1.555 |
|
|
| 50 |
1.033 |
1.067 |
1.050 |
0.911 |
|
|
| 25 |
0.591 |
0.611 |
0.601 |
0.462 |
|
|
| 12.5 |
0.380 |
0.398 |
0.389 |
0.250 |
|
|
| 6.25 |
0.278 |
0.286 |
0.282 |
0.143 |
|
|
| 3.12 |
0.203 |
0.207 |
0.205 |
0.066 |
|
|
| 0 |
0.138 |
0.139 |
0.139 |
|
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
