Rat Soluble protein-100B,S-100B ELISA Kit
Product Details
| Target Name |
S100 calcium binding protein B |
| Alternative Names |
S100b ELISA Kit; Protein S100-B ELISA Kit; S-100 protein beta chain ELISA Kit; S-100 protein subunit beta ELISA Kit; S100 calcium-binding protein B ELISA Kit |
| Abbreviation |
S100B |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
3.12 pg/mL-200 pg/mL |
| Sensitivity |
0.78 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Signal Transduction |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat S-100B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
85 |
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| Range % |
80-93 |
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| 1:2 |
Average % |
97 |
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| Range % |
92-104 |
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| 1:4 |
Average % |
94 |
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| Range % |
89-100 |
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| 1:8 |
Average % |
95 |
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| Range % |
89-101 |
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| Recovery |
| The recovery of rat S-100B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
92 |
88-96 |
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| EDTA plasma (n=4) |
89 |
84-96 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 200 |
1.954 |
1.874 |
1.914 |
1.751 |
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| 100 |
1.562 |
1.531 |
1.547 |
1.384 |
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| 50 |
1.174 |
1.168 |
1.171 |
1.008 |
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| 25 |
0.802 |
0.816 |
0.809 |
0.646 |
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| 12.5 |
0.567 |
0.552 |
0.560 |
0.397 |
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| 6.25 |
0.347 |
0.355 |
0.351 |
0.188 |
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| 3.12 |
0.264 |
0.261 |
0.263 |
0.100 |
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| 0 |
0.158 |
0.167 |
0.163 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
