Rat Stromal cell derived factor 1a,SDF-1a ELISA Kit
Product Details
| Target Name |
Stromal cell derived factor 1a,SDF-1a |
| Abbreviation |
SDF1a/CXCL12 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, cell culture supernates, tissue homogenates |
| Detection Range |
0.78 pg/mL-50 pg/mL |
| Sensitivity |
0.195 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat SDF-1a in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:100 |
Average % |
88 |
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| Range % |
83-97 |
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| 1:200 |
Average % |
97 |
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| Range % |
91-103 |
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| 1:400 |
Average % |
84 |
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| Range % |
80-88 |
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| 1:800 |
Average % |
89 |
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| Range % |
81-95 |
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| Recovery |
| The recovery of rat SDF-1a spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
94 |
89-96 |
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| EDTA plasma (n=4) |
97 |
94-100 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 50 |
2.830 |
2.775 |
2.803 |
2.666 |
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| 25 |
2.326 |
2.453 |
2.390 |
2.253 |
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| 12.5 |
1.600 |
1.632 |
1.616 |
1.479 |
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| 6.25 |
0.937 |
0.894 |
0.916 |
0.779 |
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| 3.12 |
0.528 |
0.532 |
0.530 |
0.393 |
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| 1.56 |
0.321 |
0.335 |
0.328 |
0.191 |
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| 0.78 |
0.157 |
0.148 |
0.153 |
0.016 |
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| 0 |
0.140 |
0.133 |
0.137 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
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