Rat tartrate-resistant acid phosphatase 5b,TRACP-5b ELISA Kit
Product Details
| Target Name |
tartrate-resistant acid phosphatase 5b,TRACP-5b |
| Abbreviation |
TRACP-5b |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.312 mIU/mL-20 mIU/mL |
| Sensitivity |
0.078 mIU/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Others |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TRACP-5b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
89-95 |
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| 1:2 |
Average % |
100 |
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| Range % |
97-103 |
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| 1:4 |
Average % |
93 |
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| Range % |
88-97 |
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| 1:8 |
Average % |
98 |
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| Range % |
93-102 |
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| Recovery |
| The recovery of rat TRACP-5b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
94 |
90-96 |
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| EDTA plasma (n=4) |
95 |
91-97 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| mIU/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 20 |
2.076 |
1.988 |
2.032 |
1.887 |
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| 10 |
1.292 |
1.196 |
1.244 |
1.099 |
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| 5 |
0.804 |
0.820 |
0.812 |
0.667 |
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| 2.5 |
0.511 |
0.521 |
0.516 |
0.371 |
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| 1.25 |
0.324 |
0.335 |
0.330 |
0.185 |
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| 0.625 |
0.282 |
0.278 |
0.280 |
0.135 |
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| 0.312 |
0.223 |
0.212 |
0.218 |
0.073 |
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| 0 |
0.146 |
0.144 |
0.145 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
