Rat tissue inhibitors of metalloproteinase 2,TIMP-2 ELISA Kit
Product Details
| Target Name |
TIMP metallopeptidase inhibitor 2 |
| Alternative Names |
Timp2 ELISA Kit; Timp-2 ELISA Kit; Metalloproteinase inhibitor 2 ELISA Kit; Tissue inhibitor of metalloproteinases 2 ELISA Kit; TIMP-2 ELISA Kit |
| Abbreviation |
TIMP2 |
| Uniprot No. |
P30121 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
1.25 ng/mL-80 ng/mL |
| Sensitivity |
0.31 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TIMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
91 |
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| Range % |
88-95 |
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| 1:2 |
Average % |
95 |
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| Range % |
90-100 |
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| 1:4 |
Average % |
97 |
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| Range % |
94-103 |
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| 1:8 |
Average % |
101 |
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| Range % |
96-105 |
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| Recovery |
| The recovery of rat TIMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
90 |
87-94 |
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| EDTA plasma (n=4) |
96 |
94-101 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 80 |
2.184 |
2.189 |
2.187 |
2.009 |
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| 40 |
1.486 |
1.456 |
1.471 |
1.293 |
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| 20 |
0.925 |
0.994 |
0.960 |
0.782 |
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| 10 |
0.545 |
0.588 |
0.567 |
0.389 |
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| 5 |
0.431 |
0.443 |
0.437 |
0.259 |
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| 2.5 |
0.308 |
0.292 |
0.300 |
0.122 |
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| 1.25 |
0.251 |
0.233 |
0.242 |
0.064 |
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| 0 |
0.179 |
0.177 |
0.178 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
