Name: Rat Tri-iodothyronine,T3 ELISA Kit Supplier in India | Biochain Incorporated
Brand: BioChain
Rating: 3 (19 reviews)

Product Details

Description	The sensitive quantitative measurement of total rat tr-iodothyronine (T3) in serum, plasma, cell culture supernates, or tissue homogenates is easily performed with this 96 well strip format ELISA kit. This assay employs the competitive inhibition enzyme immunoassay technique, in which T3 in the standards or the samples competes with biotin-conjugated T3 for binding to the pre-coated T3 antibody. And the color elicited by HRP-TMB chromogenic reaction develops in opposite to the amount of T3 in the sample. The color development is stopped upon the addition of the stop solution and the intensity of the color is measured by a microplate reader at 450 nm. This Human T3 ELISA Kit has high sensitivity and excellent specificity for the detection of T3. And it also has been validated with precision low than 15%, good linearity, high recovery, and lot-to-lot consistency. Refer to the product instructions for more details. T3 is a thyroid hormone that almost affects all physiological proces...Read more
Target Name	Tri-iodothyronine,T3
Abbreviation	T3
Species	Rattus norvegicus (Rat)
Sample Types	serum, plasma, cell culture supernates, tissue homogenates
Detection Range	0.5 ng/mL-8 ng/mL
Sensitivity	0.5 ng/mL
Assay Time	1-5h
Sample Volume	50-100ul
Detection Wavelength	450 nm
Research Area	Signal Transduction
Assay Principle	quantitative
Measurement	Competitive
Precision	Intra-assay Precision (Precision within an assay): CV% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV% Three samples of known concentration were tested in twenty assays to assess.
Typical Data	These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
Materials provided	A 96-well Assay plate --The 96-well plate has been pre-coated with an anti-human Ts antibody. Standard(10 x concentrate) (6 x 1 ml) --Dilute the standard at dilution series, read the OD values, and then draw a standard curve. Biotin-conjugated T3 (1 x 6 ml) HRP-avidin (1 x 6 ml) --Catalyze the TMB, eliciting a chromogenic reaction. Wash Buffer (20x concentrate) (1 x 15 ml) --Wash away unbound or free substances. Substrate A (1x 7 ml) --Mix with Substrate B. Show More
Materials not provided	A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 600 nm - 630 nm. An incubator that can provide stable incubation conditions up to 37°C±5° Centrifuge Vortex Squirt bottle, manifold dispenser, or automated microplate washer Absorbent paper for blotting the microtiter plate
Storage	Store at 2-8°C. Please refer to protocol.
Lead Time	3-5 working days

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