Rat tumor necrosis factor-inducible gene 6 protein(TSG-6) elisa kit
Product Details
| Target Name |
tumor necrosis factor-inducible gene 6 protein(TSG-6) |
| Alternative Names |
TSG-6, TSG6, hyaluronate-binding protein|tumor necrosis factor alpha-inducible protein 6|tumor necrosis factor-inducible protein 6|tumor necrosis factor-stimulated gene-6 protein |
| Abbreviation |
TSG-6 |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
0.312 ng/mL-20 ng/mL |
| Sensitivity |
0.078 ng/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
|
| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
|
|
| |
|
|
|
|
|
|
|
| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat TSG-6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| |
Sample |
Serum(n=4) |
|
| 1:1 |
Average % |
91 |
|
| Range % |
87-95 |
|
| 1:2 |
Average % |
101 |
|
| Range % |
97-104 |
|
| 1:4 |
Average % |
91 |
|
| Range % |
87-94 |
|
| 1:8 |
Average % |
100 |
|
| Range % |
96-104 |
|
|
| Recovery |
| The recovery of rat TSG-6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
|
| Serum (n=5) |
94 |
91-98 |
|
| EDTA plasma (n=4) |
94 |
91-97 |
|
| |
|
|
|
|
|
|
| |
|
|
|
|
|
|
|
| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| ng/ml |
OD1 |
OD2 |
Average |
Corrected |
|
| 20 |
2.545 |
2.532 |
2.539 |
2.373 |
|
| 10 |
1.903 |
1.867 |
1.885 |
1.719 |
|
| 5 |
1.210 |
1.222 |
1.216 |
1.050 |
|
| 2.5 |
0.683 |
0.718 |
0.701 |
0.535 |
|
| 1.25 |
0.429 |
0.448 |
0.439 |
0.273 |
|
| 0.625 |
0.341 |
0.332 |
0.337 |
0.171 |
|
| 0.312 |
0.256 |
0.243 |
0.250 |
0.084 |
|
| 0 |
0.168 |
0.164 |
0.166 |
|
|
|
|
| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
