Rat Vascular Endothelial cell Growth Factor,VEGF ELISA Kit
Product Details
| Description |
The Rat Vascular Endothelial cell Growth Factor (VEGF) ELISA Kit quantitates VEGF levels in rat serum, plasma, or tissue homogenates. VEGF is a key regulator of endothelial cell proliferation and migration and vascular permeability. As a potent pro-angiogenic factor, VEGF is capable of inducing physiological and pathological angiogenesis involved in the pathogenesis of cancerous tumors. VEGF signaling elicits key downstream signaling pathways including MAPK/ERK1/2, endothelial nitric oxide synthase (eNOS), and mammalian target of rapamycin (mTOR).
This kit employs the sandwich-ELISA mechanism in conjugation with VEGF antibody-VEGF antigen-specific binding as well as HRP-TMB chromogenic reaction to measure the concentration of VEGF in the samples. The kit is characterized by high sensitivity, strong specificity, good linearity, recovery with 90%-102%, precision less than 10%. Refer to the product instructions for more validation information.
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| Target Name |
vascular endothelial growth factor A |
| Alternative Names |
Vegfa ELISA Kit; Vegf ELISA Kit; Vascular endothelial growth factor A ELISA Kit; VEGF-A ELISA Kit; Vascular permeability factor ELISA Kit; VPF ELISA Kit |
| Abbreviation |
VEGFA |
| Species |
Rattus norvegicus (Rat) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
3.9 pg/mL-250 pg/mL |
| Sensitivity |
0.97 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Cancer |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of rat VEGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
93 |
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| Range % |
84-101 |
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| 1:2 |
Average % |
94 |
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| Range % |
90-100 |
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| 1:4 |
Average % |
96 |
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| Range % |
91-103 |
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| 1:8 |
Average % |
94 |
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| Range % |
86-100 |
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| Recovery |
| The recovery of rat VEGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
90-97 |
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| EDTA plasma (n=4) |
97 |
92-102 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 250 |
2.712 |
2.892 |
2.802 |
2.614 |
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| 125 |
2.369 |
2.317 |
2.343 |
2.155 |
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| 62.5 |
1.568 |
1.604 |
1.586 |
1.398 |
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| 31.25 |
0.877 |
0.888 |
0.883 |
0.695 |
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| 15.6 |
0.558 |
0.562 |
0.560 |
0.372 |
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| 7.8 |
0.371 |
0.384 |
0.378 |
0.190 |
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| 3.9 |
0.276 |
0.286 |
0.281 |
0.093 |
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| 0 |
0.188 |
0.187 |
0.188 |
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| Materials provided |
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-rat VEGF antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled VEGF antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody solution.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
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| Materials not provided |
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
