Product Details
| Description |
The full-length cDNA ORF of Bacillus anthracis Thymidylate kinase (tmk) was N-terminally tagged a 6xHis and then expressed in E.coli. The Recombinant Bacillus anthracis tmk obtained was purified using SDS-PAGE analysis and got over 90% of purity. Under reducing conditions, the tmk protein migrated to an approximately 28 kDa molecular mass band on the SDS-PAGE gel. This recombinant tmk protein may be used to produce specific antibodies or find uses in the DNA synthesis-related studies. Tmk is a key enzyme exerting an important role in DNA synthesis. It catalyzes the conversion of thymidine monophosphate (dTMP) to thymidine diphosphate (dTDP), with ATP as the preferred phosphoryl donor and in the presence of Mg2+. It takes place at the junction of the de novo and salvage pathways for thymidine triphosphate (dTTP) synthesis. Tmk is thus regarded as a potential therapeutic target for the development of antibacterial, antiparasitic, and anticancer drugs. |
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| Purity | Greater than 90% as determined by SDS-PAGE. | ||
| Target Names | tmk | Research Area | Others |
| Alternative Names | tmk; BAMEG_0039Thymidylate kinase; EC 2.7.4.9; dTMP kinase | ||
| Species | Bacillus anthracis (strain CDC 684 / NRRL 3495) | ||
| Source | E.coli | ||
| Expression Region | 1-208aa | ||
| Target Protein Sequence | MKGLFVTIEGPEGSGKTTLIQSLLPYFEQKEQKVMATREPGGIAISEDIRTILHKQEYTMMEARTEALLYAAARRQHLVEKVMPALNEDYLVLCDRFIDSSLAYQGYARGLGMDKVFEINRFATEDCMPSLTIYLDIEPEVGLARIAKDAGREVNRLDMEDISFHKRVREGYLQVVERFSDRIVLVNADQPMEKLIEEVIQVIEDKLL Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. |
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| Mol. Weight | 27.8kDa | ||
| Protein Length | Full Length | ||
| Tag Info | N-terminal 6xHis-tagged | ||
| Form | Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. |
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| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Note: If you have any special requirement for the glycerol content, please remark when you place the order. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
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| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. | ||
| Storage Condition | Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. | ||
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
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| Lead Time | Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time. | ||
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. | ||
| Datasheet & COA | Please contact us to get it. |