Product Details
| Description |
The sequence encoding the 1-427aa of the Clostridium botulinum Botulinum neurotoxin type B (botB) was expressed in the E.coli cells. Each product has fused a 6xHis-B2M-tag at the N-terminus. The recombinant truncated Clostridium botulinum botB protein was purified by SDS-PAGE and got a purity of over 85%. The botB protein migrated to a molecular weight of about 64 kDa on the SDS-PAGE gel. And it was also validated by the LC-MS/MS analysis. In-stock botB proteins are offered now. This recombinant botB protein may find uses as an immunogen for specific antibody synthesis or in the studies of neurological diseases. BotB is a Clostridium botulinum-expressed toxin that reduces muscle hyperactivity through its function at the neuromuscular junction. Once released into the cytosol, botB undergoes a multistep cascade of binding, internalization, cytosolic escape, and cleavage of the vesicle protein VAMP/SNAP-25 through its light chain (LV), resulting in the blockade of synaptic vesicle exocyto...Read more
|
| Purity | Greater than 85% as determined by SDS-PAGE. |
| Target Names | botB |
| Research Area | Others |
| Alternative Names | botBBotulinum neurotoxin type B; BoNT/B; Bontoxilysin-B) [Cleaved into: Botulinum neurotoxin B light chain; LC; EC 3.4.24.69); Botulinum neurotoxin B heavy chain; HC)] |
| Species | Clostridium botulinum |
| Source | E.coli |
| Expression Region | 1-427aa |
| Target Protein Sequence | MPVTINNFNYNDPIDNNNIIMMEPPFARGTGRYYKAFKITDRIWIIPERYTFGYKPEDFNKSSGIFNRDVCEYYDPDYLNTNDKKNIFLQTMIKLFNRIKSKPLGEKLLEMIINGIPYLGDRRVPLEEFNTNIASVTVNKLISNPGEVERKKGIFANLIIFGPGPVLNENETIDIGIQNHFASREGFGGIMQMKFCPEYVSVFNNVQENKGASIFNRRGYFSDPALILMHELIHVLHGLYGIKVDDLPIVPNEKKFFMQSTDAIQAEELYTFGGQDPSIITPSTDKSIYDKVLQNFRGIVDRLNKVLVCISDPNININIYKNKFKDKYKFVEDSEGKYSIDVESFDKLYKSLMFGFTETNIAENYKIKTRASYFSDSLPPVKIKNLLDNEIYTIEEGFNISDKDMEKEYRGQNKAINKQAYEEISKE Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. |
| Mol. Weight | 63.3 kDa |
| Protein Length | Partial |
| Tag Info | N-terminal 6xHis-B2M-tagged |
| Form | Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Note: If you have any special requirement for the glycerol content, please remark when you place the order. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Storage Condition | Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Lead Time | Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Datasheet & COA | Please contact us to get it. |