Product Details
| Description |
Full-length of mature human interferon-induced, double-stranded RNA-activated protein kinase EIF2AK2 cDNA carrying an N-terminal 6xHis-SUMO-tag was expressed in E.coli. The resulting protein is the recombinant fusion protein consisting of 2-551aa of human EIF2AK2. It was validated by both SDS-PAGE and LC-MS/MS analyses. The SDS-PAGE showed an about 75-85 kDa molecular mass of this protein and determines the purity of over 90%. In addition to being an immunogen for specific antibody synthesis, this recombinant protein also may be used in the studies of EIF2AK2-mediated signal transduction. EIF2AK2, also called PKR, is activated by double-stranded RNA (dsRNA) and blocks the translation of viral mRNA in response to infection and physiologic stress conditions. It is also involved in innate immune response and the modulation of signal transduction, apoptosis, cell proliferation, and differentiation. Dongxue Mao etc. demonstrated that EIF2AK2 missense variants lead to a neurodevelopmental sy...Read more
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| Purity | Greater than 90% as determined by SDS-PAGE. |
| Target Names | EIF2AK2 |
| Research Area | Signal Transduction |
| Alternative Names | Double stranded RNA activated protein kinase; E2AK2_HUMAN; eIF-2A protein kinase 2; EIF2AK1; EIF2AK2; Eukaryotic translation initiation factor 2 alpha kinase 2; Eukaryotic translation initiation factor 2-alpha kinase 2; HGNC:9437; Interferon induced double stranded RNA activated protein kinase; Interferon inducible elF2 alpha kinase ; Interferon inducible RNA dependent protein kinase; Interferon-induced; double-stranded RNA-activated protein kinase; Interferon-inducible RNA-dependent protein kinase; MGC126524; P1/eIF-2A protein kinase; P1/eIF2A protein kinase ; p68 kinase; PKR; PPP1R83; PRKR; Protein kinase interferon inducible double stranded RNA dependent; Protein kinase RNA activated; Protein kinase RNA-activated; Protein phosphatase 1 regulatory subunit 83; Serine/threonine protein kinase TIK ; Tyrosine protein kinase EIF2AK2 |
| Species | Homo sapiens (Human) |
| Source | E.coli |
| Expression Region | 2-551aa |
| Target Protein Sequence | AGDLSAGFFMEELNTYRQKQGVVLKYQELPNSGPPHDRRFTFQVIIDGREFPEGEGRSKKEAKNAAAKLAVEILNKEKKAVSPLLLTTTNSSEGLSMGNYIGLINRIAQKKRLTVNYEQCASGVHGPEGFHYKCKMGQKEYSIGTGSTKQEAKQLAAKLAYLQILSEETSVKSDYLSSGSFATTCESQSNSLVTSTLASESSSEGDFSADTSEINSNSDSLNSSSLLMNGLRNNQRKAKRSLAPRFDLPDMKETKYTVDKRFGMDFKEIELIGSGGFGQVFKAKHRIDGKTYVIKRVKYNNEKAEREVKALAKLDHVNIVHYNGCWDGFDYDPETSDDSLESSDYDPENSKNSSRSKTKCLFIQMEFCDKGTLEQWIEKRRGEKLDKVLALELFEQITKGVDYIHSKKLIHRDLKPSNIFLVDTKQVKIGDFGLVTSLKNDGKRTRSKGTLRYMSPEQISSQDYGKEVDLYALGLILAELLHVCDTAFETSKFFTDLRDGIISDIFDKKEKTLLQKLLSKKPEDRPNTSEILRTLTVWKKSPEKNERHTC Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. |
| Mol. Weight | 78.0kDa |
| Protein Length | Full Length of Mature Protein |
| Tag Info | N-terminal 6xHis-SUMO-tagged |
| Form | Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Note: If you have any special requirement for the glycerol content, please remark when you place the order. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Storage Condition | Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Lead Time | Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Datasheet & COA | Please contact us to get it. |