Product Details
| Description |
A DNA sequence encoding the 1-498 aa of Influenza A virus was expressed with an N-terminal 6xHis-tag in E.coli. The resulting protein is a recombinant full-length Influenza A virus Nucleoprotein (NP). Its identity was confirmed by the LC-MS/MS Analysis. SDS-PAGE assay determined its purity greater than 90%. This NP protein may be used for specific antibody synthesis or in the studies of the microbiology research area. Influenza A virus NP is a structural protein with the most abundant expression and no intrinsic enzymatic activity in infected cells. It mainly encapsidates the segmented RNA and then combines with the three polymerase subunits (PA, PB1, and PB2) to form the viral ribonucleoprotein particles (vRNPs) for RNA transcription ＆ replication, and packaging. Various nuclear localization signals (NLS) in the amino acid sequence of NP are responsible for the active RNP import into the nucleus. Besides, NP is one of the main determinants of influenza species specificity. The self-ol...Read more
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| Purity | Greater than 90% as determined by SDS-PAGE. |
| Target Names | NP |
| Research Area | Microbiology |
| Alternative Names | NP; Nucleoprotein; Nucleocapsid protein; Protein N |
| Species | Influenza A virus |
| Source | E.coli |
| Expression Region | 1-498aa |
| Target Protein Sequence | MASQGTKRSYEQMETDGERQNATEIRASVGKMIDGIGRFYIQMCTELKLSDYEGRLIQNSLTVERMVLSAFDERRNRYLEEHPSAGKDPKKTGGPIYKRVGGRWMRELVLYDKEEIRRIWRQANNGDDATRGLTHMMIWHSNLNDTTYQRTRALVRTGMDPRMCSLMQGSTLPRRSGAAGAAVKGIGTMVMELIRMIKRGINDRNFWRGENGRKTRSAYERMCNILKGKFQTAAQRAMMDQVRESRNPGNAEIEDLIFSARSALILRGSVAHKSCLPACVYGPAVSSGYDFEKEGYSLVGIDPFKLLQNSQVYSLIRPNENPAHKSQLVWMACHSAAFEDLRLLSFIRGTKVSPRGKLSTRGVQIASNENMDNMESSTLELRSRYWAIRTRSGGNTNQQRASAGQISVQPTFSVQRNLPFEKSTVMAAFTGNTEGRTSDMRAEIIRMMEGAKPEEVSFRGRGVFELSDEKATNPIVPSFDMSNEGSYFFGDNAEEYDN Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. |
| Mol. Weight | 60.2kDa |
| Protein Length | Full Length |
| Tag Info | N-terminal 6xHis-tagged |
| Form | Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Note: If you have any special requirement for the glycerol content, please remark when you place the order. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Storage Condition | Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Lead Time | Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Datasheet & COA | Please contact us to get it. |