Product Details
| Description |
Troponin I, cardiac muscle (Tnni3) CSB-EP341785MO is a recombinant mouse full-length of mature Tnni3 protein containing amino acid residues of 2-211. It is produced in the E.coli cells through procaryotic expression and fused with a 6xHis-SUMO-tag at the N-terminus. And it has high purity of up to 90?termined by SDS-PAGE. Its predicted molecular weight is 40.11 kDa, but the actual observed molecular mass (46 kDa)via SDS-PAGE analysis is slightly higher due to post-transcriptional modifications such as glycosylation. This recombinant Tnni3 protein is in-stock, allowing for continuous sourcing and no intermediate waiting period for protein preparation. This Tnni3 protein not only acts as an immunogen for antibody production but also finds uses in the studies of Tnni3-mediated signal transduction. Tnni3 is the inhibitory subunit of the cardiac troponin (cTn) complex that is a sensor of the intracellular Ca2+ levels and modulates the interaction between the thick and thin filaments duri...Read more
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| Purity | Greater than 90% as determined by SDS-PAGE. |
| Target Names | Tnni3 |
| Research Area | Signal Transduction |
| Alternative Names | Tnni3; Troponin I; cardiac muscle; Cardiac troponin I |
| Species | Mus musculus (Mouse) |
| Source | E.coli |
| Expression Region | 2-211aa |
| Target Protein Sequence | ADESSDAAGEPQPAPAPVRRRSSANYRAYATEPHAKKKSKISASRKLQLKTLMLQIAKQEMEREAEERRGEKGRVLRTRCQPLELDGLGFEELQDLCRQLHARVDKVDEERYDVEAKVTKNITEIADLTQKIYDLRGKFKRPTLRRVRISADAMMQALLGTRAKESLDLRAHLKQVKKEDIEKENREVGDWRKNIDALSGMEGRKKKFEG Note: The complete sequence including tag sequence, target protein sequence and linker sequence could be provided upon request. |
| Mol. Weight | 40.1kDa |
| Protein Length | Full Length of Mature Protein |
| Tag Info | N-terminal 6xHis-SUMO-tagged |
| Form | Liquid or Lyophilized powder Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. |
| Buffer | If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. Note: If you have any special requirement for the glycerol content, please remark when you place the order. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. |
| Storage Condition | Store at -20°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles. |
| Shelf Life | The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. |
| Lead Time | Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
| Notes | Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week. |
| Datasheet & COA | Please contact us to get it. |