Components:
- Reagent I: 30 mL×1, store at 4℃ .
- Reagent II: 5 mL×1, store at 4℃ .
- Reagent Ⅲ: 1 mL×1, store at 4℃ .
- Reagent ⅣA: 2.5 mL×1, store at 4℃ .
- Reagent ⅣB: 2.5 mL×1, store at 4℃ . (Mix reagents accordance the ratio A:B=1:1 before use).
- Reagent V: 12 mL×1, store at 4℃ .
- Standard: Powder×1, store at 4℃ . Add 0.9 mL of distilled water before use to prepare as 40 μmol/mL FeSO4 •7H2O, then dilute with distilled water to 0.5 μmol/mL.
Description:
Total iron-binding capacity (TIBC) refers to the ability of serum transferrin to bind iron, and its content is closely related to the diseases such as iron deficiency anemia and acute hepatitis. Fe2+ reacts with ferrozine to form a fuchsia compound which has an absorption peak at 562nm. In alkaline condition, serum transferrin can bind with Fe3+, and the remaining unbound Fe3+ can be reduced to Fe2+ . So the absorbance A1 is positively correlated with Fe3+ . After acidification, the transferrin-bound Fe3+ is released and further reduced to Fe2+ . The absorbance A2 has a positive correlation with Fe3+ , A2 minus A1 was proportional to TIBC.
