Sheep Interleukin 12,IL-12 ELISA Kit
Product Details
| Target Name |
Interleukin 12,IL-12 |
| Alternative Names |
N/A |
| Abbreviation |
IL12 |
| Species |
Ovis aries (Sheep) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
31.25 pg/mL-2000 pg/mL |
| Sensitivity |
7.81 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
90 |
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| Range % |
87-94 |
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| 1:2 |
Average % |
103 |
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| Range % |
99-107 |
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| 1:4 |
Average % |
93 |
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| Range % |
87-99 |
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| 1:8 |
Average % |
91 |
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| Range % |
88-96 |
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| Recovery |
| The recovery of sheep IL-12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
96 |
92-103 |
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| EDTA plasma (n=4) |
90 |
86-94 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 2000 |
2.599 |
2.780 |
2.690 |
2.596 |
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| 1000 |
2.177 |
2.261 |
2.219 |
2.125 |
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| 500 |
1.621 |
1.730 |
1.676 |
1.582 |
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| 250 |
1.113 |
1.056 |
1.085 |
0.991 |
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| 125 |
0.756 |
0.782 |
0.769 |
0.675 |
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| 62.5 |
0.399 |
0.411 |
0.405 |
0.311 |
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| 31.25 |
0.280 |
0.297 |
0.289 |
0.195 |
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| 0 |
0.097 |
0.090 |
0.094 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
