Sheep Interleukin 4(IL-4)ELISA Kit
Product Details
| Target Name |
interleukin 4 |
| Alternative Names |
IL4 ELISA Kit; IL-4Interleukin-4 ELISA Kit; IL-4 ELISA Kit; B-cell stimulatory factor 1 ELISA Kit; BSF-1 ELISA Kit; Lymphocyte stimulatory factor 1 ELISA Kit |
| Abbreviation |
IL4 |
| Species |
Ovis aries (Sheep) |
| Sample Types |
serum, plasma, tissue homogenates |
| Detection Range |
6.25 pg/mL-400 pg/mL |
| Sensitivity |
1.56 pg/mL |
| Assay Time |
1-5h |
| Sample Volume |
50-100ul |
| Detection Wavelength |
450 nm |
| Research Area |
Immunology |
| Assay Principle |
quantitative |
| Measurement |
Sandwich |
| Precision |
| Intra-assay Precision (Precision within an assay): CV% |
| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV% |
| Three samples of known concentration were tested in twenty assays to assess. |
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| Linearity |
| To assess the linearity of the assay, samples were spiked with high concentrations of sheep IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
92 |
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| Range % |
86-95 |
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| 1:2 |
Average % |
102 |
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| Range % |
97-105 |
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| 1:4 |
Average % |
95 |
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| Range % |
90-97 |
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| 1:8 |
Average % |
97 |
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| Range % |
93-100 |
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| Recovery |
| The recovery of sheep IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
94 |
90-97 |
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| EDTA plasma (n=4) |
96 |
90-99 |
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| Typical Data |
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 400 |
2.084 |
1.958 |
2.021 |
1.869 |
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| 200 |
1.300 |
1.276 |
1.288 |
1.136 |
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| 100 |
0.828 |
0.817 |
0.823 |
0.671 |
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| 50 |
0.518 |
0.529 |
0.524 |
0.372 |
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| 25 |
0.333 |
0.343 |
0.338 |
0.186 |
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| 12.5 |
0.281 |
0.290 |
0.286 |
0.134 |
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| 6.25 |
0.231 |
0.224 |
0.228 |
0.076 |
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| 0 |
0.154 |
0.150 |
0.152 |
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| Storage |
Store at 2-8°C. Please refer to protocol. |
| Lead Time |
3-5 working days |
